Sample requirements and shipping guidelines

3billion sample requirements are described below. Only samples meeting the requirements shall be processed for testing.

1. Sample type

Whole Blood

  • Collection:

    • Minimum 1 mL of blood
  • Unacceptable sample:

    • Hemolyzed or clotted blood
    • Blood from patients who received allogeneic hematopoietic stem cell transplant
    • Blood from patients who received any blood product containing leukocytes within 2 weeks prior to specimen collection
    • Sample is not cold enough at the time of arrival at 3billion laboratory.
    • Sample with any likely signs of contamination due to blood leaking out of the container, a damaged container, or unknown sediment present in the blood.
  • Container:

    • Pink or purple-top tubes containing EDTA
  • Labeling:

    • 3billion ID and date-of-birth (YYYY-MM-DD) should clearly be labeled on the tube
  • Shipping temperature:

    • Room temperature within 1 day after sample collection
    • We recommend including cold packs in your package if:
      • the shipment cannot arrive at 3billion overnight
      • shipping during the hot weather
      • shipping frozen blood

Buccal swab

  • Collection: (Collection kit provided by 3billion)

    • We provide buccal swab collection kits upon request. Please contact support@3billion.io to receive assistance with kit delivery. There is no additional cost for the request of our collection kits.
  • Unacceptable specimen:

    • Buccal kits not provided by 3billion will not be accepted
  • Guideline:

    • Instructions for the collection of the buccal specimen are enclosed in the kit. You must use both swabs to collect the buccal specimen, break off the tips, and insert both swabs in the provided preservation buffer container.
  • Labeling:

    • 3billion ID and date-of-birth (YYYY-MM-DD) should clearly be labeled on the tube containing the preservation buffer, not on the plastic package.
  • Shipping temperature:

    • Room temperature within 1 weeks after sample collection

DNA

  • Collection:

    • Genomic DNA (gDNA) must be extracted at a laboratory appropriately qualified (preferred CLIA-certified) for clinical testing. Samples not meeting the quality/quantity criteria as described below will not be accepted.
    • Total amount: A minimum of 2.0 µg of intact gDNA
    • Concentration: approximately 20 ng/µL
    • Volume: approximately 100 µL
    • Purity: A260/A280 1.8
    • Buffer: dissolved in TE buffer
  • Acceptable sample source:

    • The most appropriate type: DNA extracted from peripheral venous blood samples.
    • The followings are also acceptable: frozen blood, buccal swab, saliva, and cultured fibroblasts.
  • Unacceptable sample source:

    • Prenatal sampling: chorionic villus, amniocyte, amniotic fluid and products of conception or abortus.
    • Tumor tissues
    • Peripheral blood or saliva from patients with active hematologic malignancy in peripheral circulation.
    • Paraffin-embedded tissues
  • Container:

    • 1.5 mL microcentrifuge tubes (preferred) or 0.2 mL PCR tubes
  • Labeling:

    • 3billion ID and date-of-birth (YYYY-MM-DD) should clearly be labeled on the tube
  • Shipping temperature:

    • gDNA can be shipped at room temperature if the sample arrives overnight. If shipping during hot weather outside South Korea, we recommend including cold packs in the package.

2. Documents

A printed copy of the requisition form and the informed consent form must be submitted with the sample. Both can be obtained from “My Orders” page on portal in your account.

  • REQUISITION FORM

    • Signed by the ordering physician/researcher
  • INFORMED CONSENT FORM

    • All terms and conditions must be agreed by the patient.
    • Signed by the patient or the patient's guardian

3. Shipping Guildelines

Kindly note that any tax charges imposed by your country shall be borne by the ordering party.

3billion will arrange for sample pickup and delivery using FedEx. To request sample pickup and delivery please download the FedEx RPI here. Fill in the required information and send to support@3billion.io. This process may take up to 1-2 business days.

3billion accepts packages Monday through Friday. If shipping from outside South Korea, please request sample pickup for a Monday or Tuesday to prevent samples from arriving during the weekend when our office is closed.

Once the Fedex shipment is arranged by 3billion team, the local Fedex representative will contact the ordering party. 3billion will provide all necessary documents required for shipping. Once received, please prepare the following number of copies and present them upon the shipment pick up:

  • Commercial (Performa) Invoice - 3 copies
  • Declaration Form - 3 copies
  • Airwall Bill - 1 copy

Additional shipping fee may be charged after the sample arrives at 3billion if the shipping weight was more than 0.5kg. Kindly note that any tax charges imposed by your country shall be borne by the ordering party.